美国Applied Biosystems™ 7500实时荧光定量PCR系统

美国Applied Biosystems™ 7500实时荧光定量PCR系统 | 时间: December-25 14:54:34 | 分类:其它仪器

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 美国Applied Biosystems™ 7500实时荧光定量PCR系统

7500实时荧光定量PCR系统描述
The 7500 Real-Time PCR System is a powerful platform for labs requiring superior performance and maximum dye versatility. This system is a sophisticated platform for users who require extended capabilities and maximum versatility. The 3rd generation platform features an innovative optical system that enhances sensitivity and lets you access a broader range of fluorophores. The variable excitation capability allows greater sensitivity for longer wavelength (red) dyes.

• Powerful, five-color platform is calibrated for the broadest range of dyes available: FAM™⁄SYBR® Green I, VIC®⁄JOE™, NED™⁄ TAMRA™⁄ Cy3™, ROX™⁄Texas Red®, and Cy5™ dyes
• Specialized optical system enables easy and accurate calibration to new dyes without requiring the addition of new filter sets
• Advanced multi-componenting algorithm minimizes spectral crosstalk – superior for multiplexing
• User-friendly software includes plate set-up wizards, multi-plate data viewing capabilities, and advanced analysis tools to make data processing simple and straightforward

NEW - 7500 Software v2.0
Now the easy-to-use StepOne software is available for both the 7500 and 7500 Fast systems with the 7500 Software v2.0 upgrade. The 7500 Software v2.0 incorporates your favorite StepOne Software features, such as a variety of plate setup wizards, standard curve dilution and master mix recipe calculators, QC flags, data filters, and email notification when a run is finished. The 7500 Software v2.0 also includes an enhanced Gene Expression Study package and has a variety of new melting curve protocol options, including multiple peak detection, step and hold temperature control, and customizable ramp rates.

The NEW Gene Expression Study package accommodates large studies better than any other instrument software package:
• Import an unlimited number of Comparative CT (relative quantitation) files to one study
• Group samples and view data both by technical replicate group and biological replicate group
• Use any gene(s) as an endogenous control, including averaging multiple controls together
• Enter known efficiency values to be factored into the RQ results

21 CFR Part 11 Module available
The SDS v1.4 21CFRp11 Module is a powerful tool for assisting with 21CFRp11 compliance, while still offering the flexibility of user-customizable configuration settings.
• Individual user log-ins can be added for up to four user groups, each group with designated permission settings
• User-customizable permission settings include fourteen system activities, e-signature authority designation, and additional security settings to give you maximum control over your compliance efforts
• Audit trails can be enabled or disabled depending on your traceability needs
• A selection of e-signatures is available to ease e-signatures into your workflow

Supports Many Applications
Applications include gene expression analysis, pathogen quantitation, SNP genotyping, isothermal and +⁄– assays utilizing internal positive controls. To facilitate many of these applications, Applied Biosystems® provides preformulated, ready-to-use, quality-tested, TaqMan® assays for use with the 7500 system. Now you can reduce your assay optimization efforts.

Upgrade to High-Speed Thermal Cycling
An optional upgrade to the 7500 Fast System is available. This 7500 Fast System uses our master mix formulations and enables you to shorten your real-time PCR runs to as little as 30 minutes.
 
► 功能强大的五色平台已经过校准,支持最多种类的染料:FAM™/SYBR® Green I、VIC®/JOE™、ED™/TAMRA™/Cy®3、ROX™/Texas Red® 和 Cy®5 染料Applied Biosystems® 7500 实时荧光定量 PCR 系统为要求最大的染料功能多样性的实验室提供了一种可靠的平台。该平台具有灵敏的光学系统,适用于宽广光谱范围内的多种荧光染料。可变的激发波长可提高长波长 (红色) 染料的检测灵敏度。
7500 软件
7500 软件融合了多种易于使用的特性,如各种反应板设置向导、标准曲线稀释和预混液配方计算器、QC标记、数据过滤器,并可在运行完成时通过电子邮件通知。7500 软件还包括功能强大的基因表达研究软件包,并提供各种熔解曲线实验方案选项,包括多峰检测、步进式温度控制和可定制的变温速率。可提供遵循美国联邦法规第 21 章第 11 节 (21 CFR Part 11) 的模块SDS v1.4 21CFR Part 11 模块是一款功能强大的工具,它遵循美国联邦法规第 21 章第 11 节的要求,同时还可提供用户可定制的配置设置灵活性。
支持多种应用
其应用领域包括基因表达分析、病原体定量、SNP 基因分型、拷贝数变异、恒温和采用内部阳性对照的 +/–Assay。为方便上述应用领域,Life Technologies 提供了预先配制、已经过质量测试的即用型 TaqMan® Assay,可与 7500 系统配合使用。现在您即可节省在分析优化方面的精力。
升级至 7500 Fast 系统
您可以选择升级至 7500 Fast 系统。该 7500 Fast 系统采用了我们的预混液配方,使您可以将实时荧光定量 PCR 程序缩短至 30 分钟。
7500 系统包括:
► 可选择 Dell® 商用系列笔记本或配有平板显示器的台式电脑
► 化学安装试剂盒包括染料校正反应板、RNA酶 P 仪器验证反应板和应用特异的 starter kit
► 仪器操作和分析软件,以及 Primer Express®引物和探针设计软件
► 包括入门指南在内的丰富的文件集,适用于各种主要的实时荧光定量 PCR 应用领域
► 由 Life Technologies 服务工程师提供的安装服务
For Research Use Only. Not for use in diagnostic procedures.
7500实时荧光定量PCR系统规格参数:

Format:
96-well plate
Optics:
Tungsten-halogen lamp, 5 emission filters, CCD Camera, 5 excitation filters
Weight:
75 lbs
Precision:
99.7% confidence (5000 & 10000 copies)
Dimensions:
Width: 34 cm (13.39 in), Height: 49 cm (19.29 in), Depth: 45 cm (17.72 in)
Throughput:
4 to 5-96-well plates per 8-hr day
Power Limit:
950 W
Sensitivity:
1 copy of RNase P gene (human genomic DNA)
Product Size:
1 system
Dynamic Range:
9 logs
Input Voltage:
120V
Calibrated Dye:
SYBR® Green I, Cy3™, Cy5™, FAM™, JOE™, VIC™, Texas Red®, TAMRA™, ROX™, NED™
Reaction Speed:
Standard
Sample Ramp Rate:
± 1.1°C⁄sec
External Computer:
Notebook: Intel Core 2 Duo T5500 (1.66 GHz), 80 GB HD, 8X DVD, 1.0 G DDR2-533 MHz SDRAM
Peak Block Ramp Rate:
2.5°C⁄sec
Temperature Accuracy:
0.25°C (35°C to 95°C after 3 min)
Passive Reference Dye:
ROX (Separate Tube), No ROX, ROX (Pre-mixed)
Reaction Volume Range:
20-100 µl
Software Compatibility:
Primer Express, 7500 System Software (RQ included), 21 CFR Part 11 Module (optional)
Temperature Uniformity:
0.50°C (after 30 sec)
Thermal Cycling System:
Peltier-Based System
For Use With (Equipment):
7500 System
Temperature Range (Metric):
4-100°C
High Throughput Compatibility:
Multiplexing

7500 实时荧光定量PCR系统订购信息

产品名称
订货编号
数量单位
7500 Fast System Upgrade Kit
4362143
1 台仪器
7500 Real-Time PCR System with Dell™ Notebook
4351104
1 台仪器
7500 Real-Time PCR System with Dell™ Tower
4351105
1 台仪器
Notebook Computer Kit for 7300/7500/7500 Fast Real-Time PCR Systems
4359286
1 套
Tower Computer Kit for 7300/7500/7500 Fast Real-Time PCR Systems
4359284
1 套
7500 System SDS v1.4 21 CFR Part 11 Module
4377354
1 套

7500荧光定量PCR仪订购信息
产品描述
货号
数量
7500实时荧光定量PCR扩增仪(配备笔记本电脑)
4351104
1台
7500实时荧光定量PCR扩增仪(配备台式电脑)
4351105
1台
7500实时荧光定量PCR扩增仪快速模式升级
4362143
1个
7500实时荧光定量PCR扩增仪SDS软件21 CFR Part 11 模块
4377354
1个
7500Fast荧光定量PCR仪订购信息

产品描述
货号
数量
新型高分辨率熔解曲线(HRM)软件
4397808
1套
7500快速实时荧光定量PCR扩增仪(配备笔记本电脑)
4351106
1台
7500快速实时荧光定量PCR扩增仪(配备台式电脑)
4351107
1台
7500实时荧光定量PCR扩增仪快速模式升级
4362143
1台
7500快速实时荧光定量PCR扩增仪SDS软件21 CFR Part 11 模块
4377355
1套

本文标题: 美国Applied Biosystems™ 7500实时荧光定量PCR系统 | Tags:美国Applied Biosystems™ 7500实时荧光定量PCR系统